Journal: BMC Veterinary Research

Article Title: Effects of artichoke ( Cynara scolymus ) polyphenolic extract on growth, antioxidants and some immune parameters in common carp ( Cyprinus carpio )

doi: 10.1186/s12917-025-04619-w

Figure Lengend Snippet: Effect of different levels of ALE on serum antioxidant enzymes activity of C. Carpio

Article Snippet: Employing a commercial kit, the serum activity of catalase (CAT) (ZellBio GmbH, Germany, E-BC-K031-S), superoxide dismutase (SOD), (ZellBio GmbH, Germany, ZX-44108-96) and glutathione peroxidase (GPx) (ZellBio GmbH, Germany, E-BC-K096-M) was measured according to the manufacturer’s protocol [ ].

Techniques: Activity Assay

Journal: Genome Biology

Article Title: The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins

doi: 10.1186/gb-2006-7-9-r80

Figure Lengend Snippet: Urinary protein separation by one-dimensional SDS gel and reverse-phase HPLC. (a) 150 μg urinary protein (25 μg/lane) from single sample and pooled sample were applied on a 4-12% Bis-Tris gel. Gel was stained by colloidal Coomassie and cut into 14 pieces (in-gel 1 set) or 10 pieces (in-gel 2 set) for single urine sample, and cut into 10 pieces for pooled urine sample. (b) 250 μg of urinary protein was applied to Vivapure Anti-HSA Kit to deplete serum albumin. The albumin-depleted protein mixture was dissolved in 6 mol/l urea and 1.0% acetic acid solution, and separated on mRP-C18 High-Recovery protein column at 80°C using linear multi-segment gradient, as described in the Materials and Methods section. HPLC, high-performance liquid chromatography; SDS, sodium dodecyl sulfate.

Article Snippet: Protein (250 μg) was applied to Vivapure Anti-HSA Kit (Vivascience, Hanover, Germany) to deplete serum albumin.

Techniques: SDS-Gel, Staining, High Performance Liquid Chromatography